ctb solution Search Results


1% ctb solution  (List Biological Laboratories)
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List Biological Laboratories 1% ctb solution
1% Ctb Solution, supplied by List Biological Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cholera toxin subunit b ctb 0.5  (List Biological Laboratories)
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List Biological Laboratories cholera toxin subunit b ctb 0.5
The <t>retrograde</t> tracer, CTb, was microinjected to the vl PAG (shown in A). In photo B, HCRT immunoreactive neurons are identified in blue and some of these were also positive for the retrograde tracer (arrowheads in photo B) indicating projection of HCRT neurons to the vl PAG. Photo C indicates that the vl PAG area where the GFP neurons (green) are located is traversed by hypocretin fibers and terminals (red). The image in D shows the presence of hypocretin-2 receptor immunoreactivity (red) in the GFP neurons (as shown by the filled arrowheads) in the vl PAG area. The unfilled arrowhead in D shows the presence of HCRT-2 receptor immunoreactivity in non-GFP labeled neurons. Scale in A = 100 µm, B = 25 µm, C = 10 µm, D = 5 µm.
Cholera Toxin Subunit B Ctb 0.5, supplied by List Biological Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cholera toxin b subunit (ctb) solution  (List Biological Laboratories)
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List Biological Laboratories cholera toxin b subunit (ctb) solution
The <t>retrograde</t> tracer, CTb, was microinjected to the vl PAG (shown in A). In photo B, HCRT immunoreactive neurons are identified in blue and some of these were also positive for the retrograde tracer (arrowheads in photo B) indicating projection of HCRT neurons to the vl PAG. Photo C indicates that the vl PAG area where the GFP neurons (green) are located is traversed by hypocretin fibers and terminals (red). The image in D shows the presence of hypocretin-2 receptor immunoreactivity (red) in the GFP neurons (as shown by the filled arrowheads) in the vl PAG area. The unfilled arrowhead in D shows the presence of HCRT-2 receptor immunoreactivity in non-GFP labeled neurons. Scale in A = 100 µm, B = 25 µm, C = 10 µm, D = 5 µm.
Cholera Toxin B Subunit (Ctb) Solution, supplied by List Biological Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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solution of dialysed 4% ctb in pbs  (List Biological Laboratories)
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List Biological Laboratories solution of dialysed 4% ctb in pbs
The <t>retrograde</t> tracer, CTb, was microinjected to the vl PAG (shown in A). In photo B, HCRT immunoreactive neurons are identified in blue and some of these were also positive for the retrograde tracer (arrowheads in photo B) indicating projection of HCRT neurons to the vl PAG. Photo C indicates that the vl PAG area where the GFP neurons (green) are located is traversed by hypocretin fibers and terminals (red). The image in D shows the presence of hypocretin-2 receptor immunoreactivity (red) in the GFP neurons (as shown by the filled arrowheads) in the vl PAG area. The unfilled arrowhead in D shows the presence of HCRT-2 receptor immunoreactivity in non-GFP labeled neurons. Scale in A = 100 µm, B = 25 µm, C = 10 µm, D = 5 µm.
Solution Of Dialysed 4% Ctb In Pbs, supplied by List Biological Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ctb solution #104  (List Biological Laboratories)
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List Biological Laboratories ctb solution #104
The <t>retrograde</t> tracer, CTb, was microinjected to the vl PAG (shown in A). In photo B, HCRT immunoreactive neurons are identified in blue and some of these were also positive for the retrograde tracer (arrowheads in photo B) indicating projection of HCRT neurons to the vl PAG. Photo C indicates that the vl PAG area where the GFP neurons (green) are located is traversed by hypocretin fibers and terminals (red). The image in D shows the presence of hypocretin-2 receptor immunoreactivity (red) in the GFP neurons (as shown by the filled arrowheads) in the vl PAG area. The unfilled arrowhead in D shows the presence of HCRT-2 receptor immunoreactivity in non-GFP labeled neurons. Scale in A = 100 µm, B = 25 µm, C = 10 µm, D = 5 µm.
Ctb Solution #104, supplied by List Biological Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ctb aqueous solution  (List Biological Laboratories)
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List Biological Laboratories ctb aqueous solution
The <t>retrograde</t> tracer, CTb, was microinjected to the vl PAG (shown in A). In photo B, HCRT immunoreactive neurons are identified in blue and some of these were also positive for the retrograde tracer (arrowheads in photo B) indicating projection of HCRT neurons to the vl PAG. Photo C indicates that the vl PAG area where the GFP neurons (green) are located is traversed by hypocretin fibers and terminals (red). The image in D shows the presence of hypocretin-2 receptor immunoreactivity (red) in the GFP neurons (as shown by the filled arrowheads) in the vl PAG area. The unfilled arrowhead in D shows the presence of HCRT-2 receptor immunoreactivity in non-GFP labeled neurons. Scale in A = 100 µm, B = 25 µm, C = 10 µm, D = 5 µm.
Ctb Aqueous Solution, supplied by List Biological Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyclonal goat anti-ctb solution product 703  (List Biological Laboratories)
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List Biological Laboratories polyclonal goat anti-ctb solution product 703
The <t>retrograde</t> tracer, CTb, was microinjected to the vl PAG (shown in A). In photo B, HCRT immunoreactive neurons are identified in blue and some of these were also positive for the retrograde tracer (arrowheads in photo B) indicating projection of HCRT neurons to the vl PAG. Photo C indicates that the vl PAG area where the GFP neurons (green) are located is traversed by hypocretin fibers and terminals (red). The image in D shows the presence of hypocretin-2 receptor immunoreactivity (red) in the GFP neurons (as shown by the filled arrowheads) in the vl PAG area. The unfilled arrowhead in D shows the presence of HCRT-2 receptor immunoreactivity in non-GFP labeled neurons. Scale in A = 100 µm, B = 25 µm, C = 10 µm, D = 5 µm.
Polyclonal Goat Anti Ctb Solution Product 703, supplied by List Biological Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reconstituted ctb solution  (List Biological Laboratories)
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List Biological Laboratories reconstituted ctb solution
The <t>retrograde</t> tracer, CTb, was microinjected to the vl PAG (shown in A). In photo B, HCRT immunoreactive neurons are identified in blue and some of these were also positive for the retrograde tracer (arrowheads in photo B) indicating projection of HCRT neurons to the vl PAG. Photo C indicates that the vl PAG area where the GFP neurons (green) are located is traversed by hypocretin fibers and terminals (red). The image in D shows the presence of hypocretin-2 receptor immunoreactivity (red) in the GFP neurons (as shown by the filled arrowheads) in the vl PAG area. The unfilled arrowhead in D shows the presence of HCRT-2 receptor immunoreactivity in non-GFP labeled neurons. Scale in A = 100 µm, B = 25 µm, C = 10 µm, D = 5 µm.
Reconstituted Ctb Solution, supplied by List Biological Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The retrograde tracer, CTb, was microinjected to the vl PAG (shown in A). In photo B, HCRT immunoreactive neurons are identified in blue and some of these were also positive for the retrograde tracer (arrowheads in photo B) indicating projection of HCRT neurons to the vl PAG. Photo C indicates that the vl PAG area where the GFP neurons (green) are located is traversed by hypocretin fibers and terminals (red). The image in D shows the presence of hypocretin-2 receptor immunoreactivity (red) in the GFP neurons (as shown by the filled arrowheads) in the vl PAG area. The unfilled arrowhead in D shows the presence of HCRT-2 receptor immunoreactivity in non-GFP labeled neurons. Scale in A = 100 µm, B = 25 µm, C = 10 µm, D = 5 µm.

Journal: PLoS ONE

Article Title: Hypocretin-2 Saporin Lesions of the Ventrolateral Periaquaductal Gray ( vl PAG) Increase REM Sleep in Hypocretin Knockout Mice

doi: 10.1371/journal.pone.0006346

Figure Lengend Snippet: The retrograde tracer, CTb, was microinjected to the vl PAG (shown in A). In photo B, HCRT immunoreactive neurons are identified in blue and some of these were also positive for the retrograde tracer (arrowheads in photo B) indicating projection of HCRT neurons to the vl PAG. Photo C indicates that the vl PAG area where the GFP neurons (green) are located is traversed by hypocretin fibers and terminals (red). The image in D shows the presence of hypocretin-2 receptor immunoreactivity (red) in the GFP neurons (as shown by the filled arrowheads) in the vl PAG area. The unfilled arrowhead in D shows the presence of HCRT-2 receptor immunoreactivity in non-GFP labeled neurons. Scale in A = 100 µm, B = 25 µm, C = 10 µm, D = 5 µm.

Article Snippet: To investigate the areas projecting to the vl PAG, the retrograde tracer, cholera toxin subunit b (23 nl; CTb 0.5%; List Biological Laboratories Inc.) was injected in the vl PAG in some of the mice (FVB-GFP; n = 8).

Techniques: Labeling

An injection of 23 nl of 0.5% CTB in vl PAG (shown the A') resulted in the retrolabeling of cells in the following areas: A- Basal forebrain (Medial preoptic area): B- Basal forebrain (magnocellular preoptic area); C- Perirhinal cortex; D- ventro-medial hypothamalus; E- lateral hypothalamus; F- tuberomammillary nucleus in the posterior hypothalamus and G- lateral pontine tegmentum. A caudal spread of injection to the locus coeruleus was seen in two cases (shown in H') and it resulted in retrograde labeling of cells in the amygdala as shown in H. Scale in A' and H' = 200 µm; A-H scale = 100 µm.Abbreviations: 3v- third ventricle; 4v- fourth ventricle; aq- aquaduct; CeA- central nucleus of Amygdala; Cpu- caudate putamen; fx- fornix; GP- globus pallidus; HDB- horizontal limb of the diagonal band of broca; LC- locus coeruleus; LDT- dorsolateral tegmentum; LDTV- ventral dorsolateral tegmentum; LH- lateral hypothalamus; LPT- lateral pontine tegmentum; MCPO- magnocellular preoptic area; MPO- medial preoptic; ns- nigrostriatal bundle; opt- optic tract; PH- posterior hypothalamus; Pir- Piriform cortex; PRh- Perirhinal cortex; SCN- suprachiasmatic nucleus; scp- superior cerebellar peduncle; SI- substantia innominata; TMN- tuberomammillary nucleus; vl PAG- ventrolateral preoptic area; VMH- ventromedial hypothalamus.

Journal: PLoS ONE

Article Title: Hypocretin-2 Saporin Lesions of the Ventrolateral Periaquaductal Gray ( vl PAG) Increase REM Sleep in Hypocretin Knockout Mice

doi: 10.1371/journal.pone.0006346

Figure Lengend Snippet: An injection of 23 nl of 0.5% CTB in vl PAG (shown the A') resulted in the retrolabeling of cells in the following areas: A- Basal forebrain (Medial preoptic area): B- Basal forebrain (magnocellular preoptic area); C- Perirhinal cortex; D- ventro-medial hypothamalus; E- lateral hypothalamus; F- tuberomammillary nucleus in the posterior hypothalamus and G- lateral pontine tegmentum. A caudal spread of injection to the locus coeruleus was seen in two cases (shown in H') and it resulted in retrograde labeling of cells in the amygdala as shown in H. Scale in A' and H' = 200 µm; A-H scale = 100 µm.Abbreviations: 3v- third ventricle; 4v- fourth ventricle; aq- aquaduct; CeA- central nucleus of Amygdala; Cpu- caudate putamen; fx- fornix; GP- globus pallidus; HDB- horizontal limb of the diagonal band of broca; LC- locus coeruleus; LDT- dorsolateral tegmentum; LDTV- ventral dorsolateral tegmentum; LH- lateral hypothalamus; LPT- lateral pontine tegmentum; MCPO- magnocellular preoptic area; MPO- medial preoptic; ns- nigrostriatal bundle; opt- optic tract; PH- posterior hypothalamus; Pir- Piriform cortex; PRh- Perirhinal cortex; SCN- suprachiasmatic nucleus; scp- superior cerebellar peduncle; SI- substantia innominata; TMN- tuberomammillary nucleus; vl PAG- ventrolateral preoptic area; VMH- ventromedial hypothalamus.

Article Snippet: To investigate the areas projecting to the vl PAG, the retrograde tracer, cholera toxin subunit b (23 nl; CTb 0.5%; List Biological Laboratories Inc.) was injected in the vl PAG in some of the mice (FVB-GFP; n = 8).

Techniques: Injection, Labeling